formamidopyrimidine glycosylase  (New England Biolabs)

Journal: Food Chemistry: X

Article Title: Impact of in vitro digestion on the bioaccessibility, genotoxicity and mutagenicity of mycotoxins in a complex Alternaria extract ☆

doi: 10.1016/j.fochx.2026.103481

Figure Lengend Snippet: Influence of in vitro digestion and food matrix on the genotoxicity of the Alternaria mycotoxin extract (15 μg/mL) in Caco-2 cells. Changes in the genotoxic properties of the CE were evaluated by applying the comet assay in the presence and absence of formamidopyrimidine-DNA glycosylase (FPG). Results are expressed as tail intensity (%) after 1 h of incubation. Exposure to UV-B radiation for 1 min served as the positive control. Dark and light blue bars indicate treatments without and with FPG, respectively. Statistical significance between non-FPG-treated samples and the solvent control (* p < 0.05, ** p < 0.01, *** p < 0.001), FPG-treated samples and the solvent control (#p < 0.05, ##p < 0.01, ###p < 0.001), and between treatments with and without FPG (within each sample; §p < 0.05, §§p < 0.01, §§§p < 0.001) was evaluated using Student's t -test. One-way ANOVA followed by Fisher's LSD post hoc test was used to evaluate differences across all conditions. Significant differences among FPG-treated samples are marked with lowercase letters, while capital letters denote differences among non-FPG-treated samples. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Formamidopyrimidine DNA glycosylase (FPG) enzyme was supplied by New England Biolabs (Frankfurt, Germany), whereas dimethyl sulfoxide (DMSO) and ethidium bromide were purchased from Carl Roth GmbH + Co. KG (Karlsruhe, Germany).

Techniques: In Vitro, Single Cell Gel Electrophoresis, Incubation, Positive Control, Solvent, Control